PCR Supplies
Detroit R & D DNA DAMAGE ANALYSIS KIT
Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
AURORA BIOLABS LLC Kras Q61R–cRAF Binding Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that regulates key cellular processes such as proliferation, differentiation, and survival through the RAS-RAF signaling pathway. Activation of KRAS occurs when GDP is exchanged for GTP, enabling interaction with downstream effectors including cRAF. Mutations in KRAS are frequently associated with human cancers, making the KRAS-cRAF interaction an important target for therapeutic development. The KRAS (Q61R)-cRAF Binding Assay Kit is a TR-FRET–based assay designed to measure the interaction between activated KRAS and cRAF. In this assay, Tag2-KRAS (Q61R) is loaded with GppNHp to mimic the active GTP-bound state, while Tag1-cRAF RBD binds activated KRAS. Binding generates a FRET signal detected by fluorescence emission at 665 nm and 620 nm. Inhibitors that disrupt KRAS-cRAF binding reduce the HTRF signal intensity.
Alkali Scientific Radiant™ Probe 1-Step Lo-ROX qPCR Kit, 300 Reactions, (3 X 1mL Probe 1-Step Lo-ROX 2X qPCR Mix, 3 x 0.2mL 20X RTase with RNase Inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ Probe No-ROX qPCR Kit, 500 Reactions, (5 x 1mL)
Radiant™ Probe No-ROX qPCR Kit, 500 Reactions, (5 x 1mL)
Alkali Scientific Radiant™ 2X Taq Mastermix 1000 Reactions 25 x 1mL
Radiant™ 2X Taq Mastermix 1000 Reactions 25 x 1mL
New England Biolabs, Inc. OneTaq Quick-Load 2X Master Mix with Standard Buffer – 500 reactions (50 µl vol)
OneTaq Quick-Load 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The convenient quick-load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.
GenDEPOT OLIGO DT/RANDOM HEXAMER 50RXNS
amfiRivert cDNA Synthesis Platinum Master Mix, Oligo dT/Random Hexamer, 50 rxns
Medchemexpress LLC KRas G12R inhibitor 1 | C39H38ClF7N6O9 | 1 MG
KRas G12R inhibitor 1 is a covalent inhibitor that specifically targets the oncogenic mutant KRas G12R. It functions by exploiting the strong nucleophilicity of the mutant cysteine and irreversibly binding in the Switch II region of KRas, due to its α,β-diketoamide structure. This product can be used in research for K-Ras (G12R)-driven cancers, such as pancreatic ductal adenocarcinoma (PDAC).
- Covalent inhibitor targeting KRas G12R mutant
- Selectivity for the mutant arginine
- Irreversibly binds in the Switch II region of KRas
- Possesses an α,β-diketoamide structure
- Potential for research in K-Ras (G12R)-driven cancers like pancreatic ductal adenocarcinoma (PDAC)
- Purity: 98.40%
- Appearance: Solid, white to off-white
Cayman Chemical GR Sample Buffer10X 1ea
An alkaloid with anti-inflammatory activity reduces LPS-induced TNF-a release from and NO production in RAW 264.7 macrophages at 10 30 and 100 UM reduces paw swelling in a rat model of CFA-induced arthritis at 9 and 27 mg/kg reduces colonic inflammatory cytokine levels and maintains mucosal barrier function in a mouse model of DSS-induced colitis from 25-100 mg/kg
ACROBiosystems E1A&SV40LTA resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Apexbio Technology LLC 1STEP COMP CELL PREP KIT 200T
1STEP COMP CELL PREP KIT 200T APEXBIO TECHNOLOGIES